CLONING AN AUTONOMOUS REPLICATION SEQUENCE FROM ASPERGILLUS-ORYZAE

DNA sequence from Aspergillus oryzae genome which, confers on pOK-1 th e ability to replicate autonomously in Saccharomyces cerevisiae is des cribed. A hybrid plasmid pOK-1 was constructed from pBR325 and YEp13 f ragment containing Leu2+ gene. Asp. oryzae DNA and pOK-1 were cleaved with BamHI, ligated and introduced into Escherichia coli. About 600 re combinant plasmids were obtained. One of them transforms S. cerevisiae SHY3 to Leu2+ with middle efficiency. The plasmid designated as pSB1- 2 contained two BamHI fragments (4.3 kb and 4.1 kb) of Asp. oryzae DNA .