Te. Pearce et al., UNIFORM DETECTION OF HIV-1 IN ALVEOLAR MACROPHAGES OF PEDIATRIC BUT NOT ADULT AIDS PATIENTS, Journal of leukocyte biology, 53(6), 1993, pp. 722-726
Manifestations of pulmonary disease in pediatric AIDS patients differ
from those in adults. To evaluate whether differences in the frequency
of alveolar macrophage (AM) infection with human immunodeficiency vir
us type 1 (HIV-1) could account for these clinical distinctions, we un
dertook a comparative analysis of HIV-1 DNA in AMs from pediatric and
adult AIDS patients by enzymatic amplification. A higher frequency of
viral DNA detection in pediatric cases (100%) compared with adults (67
%) was observed. The sensitivity of detection was 1 viral DNA copy per
4000 AMs; matched peripheral blood mononuclear cells from six of seve
n pediatric and eight of nine adult patients tested HIV-1 DNA positive
. Adult but not pediatric patients exhibited a marked alveolar lymphoc
ytosis, 32% mean lymphocyte count compared with 7.0%, respectively. Th
ese results suggest that the burden of HIV-1 in the lungs of pediatric
AIDS patients is greater than that in adults.