ISOLATION OF HUMAN VENTRICULAR AND ATRIAL CARDIOMYOCYTES - TECHNICAL NOTE

Techniques suitable for the isolation of human cardiac cells have prev iously been reported. However, there are difficulties concerning the r eliability and reproducibility of the available methods. In the presen t study, a technique developed for the isolation of canine cardiocytes was successfully applied to allow isolation of human atrial and ventr icular myocytes from small samples of myocardium. Application of this method allowed a direct comparison of the ionic currents recorded from human atrial and ventricular myocytes with those recorded from other mammalian preparations.