C. Guarnieri et al., AGE-RELATED-CHANGES IN CARDIAC MITOCHONDRIAL ENERGETICS UNDER THE INFLUENCE OF CALCIUM IN RAT, Cardioscience, 4(2), 1993, pp. 117-120
Mitochondria extracted from the hearts of Wistar rats aged 6 and 24 mo
nths showed similar values for the respiratory control index (RCI), st
ate 3 oxygen consumption (QO2) and ADP/O measured using glutamate or s
uccinate as substrates; with the exception that the QO2 of the aged ra
ts was lower than that of the young rats in the presence of glutamate.
The consumption of O2 induced by 2-oxoglutarate and ADP was similar i
n both age groups. Concentrations of external free Ca2+ ranging from 0
.2 to 0.8 muM produced an increase in O2 consumption and ATP formation
in the young mitochondria, with a maximum effect at 0.2 muM external
free Ca2+. Little or no change in O2 consumption and ATP formation was
evident in aged mitochondria following incubations in concentrations
of external free Ca2+ ranging from 0.2 to 0.8 muM. The continuous rate
of formation of ATP, measured in the presence of 0.2 muM external fre
e Ca2+ using a luminescence method, confirmed the previous results. Th
is study indicates that the cardiac mitochondrial phosphorylating syst
em of aged rats is poorly sensitive to variations in external free cal
cium.