CONTRIBUTION OF OCCUPATION AND DIET TO WHITE BLOOD-CELL POLYCYCLIC AROMATIC HYDROCARBON-DNA ADDUCTS IN WILDLAND FIREFIGHTERS

Wildland (forest) firefighters are exposed to a wide range of carcinog enic polycyclic aromatic hydrocarbons (PAH) in forest fire smoke. PAH undergo metabolic activation and can subsequently bind to DNA. In this study, we investigated the association between occupational and dieta ry PAH exposures and the formation of WBC PAH-DNA adducts in a populat ion of wildland firefighters. An enzyme-linked immunosorbent assay usi ng an antiserum elicited against benzo(a)pyrene-modified DNA was used to measure PAH-DNA adducts in WBC obtained from 47 California firefigh ters at two time points, early and late in the 1988 forest fire season . PAH-DNA adduct levels were not associated with cumulative hours of r ecent firefighting activity. However, firefighters who consumed charbr oiled food within the previous week had elevated PAH-DNA adduct levels , which were related to frequency of charbroiled food intake. These fi ndings suggest that dietary sources of PAH contribute to PAH-DNA adduc t levels in peripheral WBC and should be evaluated when using this ass ay to assess occupational and environmental PAH exposure.