PRODUCTION OF PSEUDORABIES VIRUS GROWTH-FACTOR (PRGF) IN THE PRESENCEOF VARIOUS MONOSACCHARIDES AND CATIONS

Pseudorabiesvirus (PRV) growth factor (PRGF), which transforms normal and represses the transformed phenotype of transformed cells, is norma lly produced in PRV infected cells fed with glucose. So prepared PRGF consist of two components (PRGF(A), PRGF(B)). The replacement of gluco se by other monosaccharides leads to three outcomes: 1. Production of normal, fully functional PRGF; 2. production of PRGF with the one-comp onent character, retaining transforming and devoid of transformation r epressing activity; 3. complete inhibition of PRGF production. Further more, the production of PRGF was shown to depend on the presence of Ca 2+ ions, however, it was not influenced by Mg2+ ions.