IMMOBILIZATION AND ACTIVITY OF CONCANAVALIN-A ON TANTALUM PENTOXIDE AND SILICON DIOXIDE SURFACES

Authors
KREMER FJB ENGBERSEN JFJ KRUISE J BERGVELD P STARMANS DAJ FEIJEN J REINHOUDT DN
Citation
Fjb. Kremer et al., IMMOBILIZATION AND ACTIVITY OF CONCANAVALIN-A ON TANTALUM PENTOXIDE AND SILICON DIOXIDE SURFACES, Sensors and actuators. B, Chemical, 13(1-3), 1993, pp. 176-179
Citations number
8
Categorie Soggetti
Engineering, Eletrical & Electronic","Instument & Instrumentation
Journal title
Sensors and actuators. B, Chemical → ACNP
ISSN journal
09254005
Volume
13
Issue
1-3
Year of publication
1993
Pages
176 - 179
Database
ISI
SICI code
0925-4005(1993)13:1-3<176:IAAOCO>2.0.ZU;2-5
Abstract
The physical adsorption and chemical immobilization of Concanavalin A (Con A) on semiconductor surfaces SiO2 and Ta2O5 are investigated with the help of radioactive-labelled Con A. It is shown that Con A easily forms multi-layered systems and that SiO2 has preference for the non- labelled protein. Surface coverage by the two methods is compared with respect to stability to mechanical stress and clearly shows the drawb ack of physical adsorption. In addition, the complexing abilities of C on A for mannose are investigated showing 50% activity compared to Con A in solution for monolayer systems, increasing to 75% for multi-laye red systems.