ELECTROCHEMICAL LUMINESCENCE-BASED HOMOGENEOUS IMMUNOSENSOR

Authors
TANAKA M KAMIYA S SHIBUE A NAMBA K IKARIYAMA Y AIZAWA M
Citation
M. Tanaka et al., ELECTROCHEMICAL LUMINESCENCE-BASED HOMOGENEOUS IMMUNOSENSOR, Sensors and actuators. B, Chemical, 13(1-3), 1993, pp. 184-187
Citations number
7
Categorie Soggetti
Engineering, Eletrical & Electronic","Instument & Instrumentation
Journal title
Sensors and actuators. B, Chemical → ACNP
ISSN journal
09254005
Volume
13
Issue
1-3
Year of publication
1993
Pages
184 - 187
Database
ISI
SICI code
0925-4005(1993)13:1-3<184:ELHI>2.0.ZU;2-X
Abstract
Luminol capable of generating electrochemical luminescence was employe d as a label of immunoassay. A luminol-labeled antibody generated lumi nescence upon anodic oxidation and the luminescence decreased markedly in the presence of antigen. The decrease was correlated to the antige n concentration as low as 10(-11) g/ml antigen. The proposed method wa s operated in an optoelectrochemical cell. A miniaturized and sensitiv e immunosensing can be developed by fabricating a small cell with a ve ry thin layer.