DEVELOPMENT OF RAPID DETECTION SYSTEM FOR ALLERGIC REACTION USING RATBASOPHILIC LEUKEMIA (RBL-1) CELLS

We have investigated the electrochemical detection system of allergic reaction using rat basophilic leukemia (RBL-1) cells by applying cycli c voltammetry. When the potential of the electrode is run in the range 0-1.0 V versus a saturated calomel electrode (SCE), an anodic peak cu rrent appears around 0.34 V. This peak current of RBL-1 cells is attri buted to intracellular serotonin. After the addition of allergen, this peak current increases with time and the maximum current (0.82 muA pe r 2 X 10(5) cells) is obtained after 20 min. On the other hand, the cu rrent is constant when the allergen is not added. This current increas e is attributed to localization of the intracellular serotonin and is specific for allergen.