A METHOD FOR THE ASSAY OF HYDROLYTIC ENZYMES USING DYNAMIC LIGHT-SCATTERING

Authors
WEETALL HH GAIGALAS AK
Citation
Hh. Weetall et Ak. Gaigalas, A METHOD FOR THE ASSAY OF HYDROLYTIC ENZYMES USING DYNAMIC LIGHT-SCATTERING, Applied biochemistry and biotechnology, 41(1-2), 1993, pp. 139-144
Citations number
NO
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
41
Issue
1-2
Year of publication
1993
Pages
139 - 144
Database
ISI
SICI code
0273-2289(1993)41:1-2<139:AMFTAO>2.0.ZU;2-Y
Abstract
Dynamic light-scattering techniques have been successfully used for th e assay of several hydrolytic enzymes. The enzymes were assayed using substrate-coated colloidal particles. Hydrolysis of the substrate coat causes destabilization of the particles followed by particle aggregat ion. The rate of particle aggregation can be related to the initial co ncentration of added enzyme.