Hh. Weetall et Ak. Gaigalas, A METHOD FOR THE ASSAY OF HYDROLYTIC ENZYMES USING DYNAMIC LIGHT-SCATTERING, Applied biochemistry and biotechnology, 41(1-2), 1993, pp. 139-144
Dynamic light-scattering techniques have been successfully used for th
e assay of several hydrolytic enzymes. The enzymes were assayed using
substrate-coated colloidal particles. Hydrolysis of the substrate coat
causes destabilization of the particles followed by particle aggregat
ion. The rate of particle aggregation can be related to the initial co
ncentration of added enzyme.