Although N, especially mixtures of NO3 and NH4, can increase the produ
ctivity of maize (Zea mays L.), it is unclear when this N needs to be
available for maximum benefit. This study was conducted to determine t
he importance of preanthesis N form and the need for available postant
hesis N on the productivity of maize. Four single-cross hybrids (B73 x
LH51, LH74 x LH51, LH74 x LH82, and LHE136 x LH82) were grown in fiel
d-hydroponics and supplied N as either all NO3 or an equal mixture of
NO3 and NH4 (mixed N nutrition) until anthesis, whereupon N was withdr
awn from one-half of the plants. At physiological maturity, whole shoo
ts were harvested, separated into component fractions, weighed, and an
alyzed for yield, yield components, and macronutrient composition. Com
pared with all NO3, mixed N nutrition increased grain yield of all hyb
rids whether the N was available full season (average of 11%) or only
during vegetative growth (average of 10%). In contrast, regardless of
the N form, N deprivation during grain fill decreased grain yield for
three of the four hybrids (range of 6 to 19%) but increased yield (5%)
for LHE136 x LH82. Changes in sink-source partitioning, kernel number
, and plant N accumulation were closely related to both yield enhancem
ent associated with mixed N nutrition, and yield reduction due to N re
moval. Therefore, these data show that N form, timing of N availabilit
y and genotype are all factors that interact to influence N use and pr
oductivity of maize.