THE USE OF A 3-FACTOR STATISTICAL MIXTURE DESIGN AND A 2-FACTOR CHROMATOGRAPHIC RESPONSE FUNCTION TO THE RP-HPLC SEPARATION OF SEVERAL FREEALPHA-AMINO-ACIDS

Authors
QIU HC WALTERS FH
Citation
Hc. Qiu et Fh. Walters, THE USE OF A 3-FACTOR STATISTICAL MIXTURE DESIGN AND A 2-FACTOR CHROMATOGRAPHIC RESPONSE FUNCTION TO THE RP-HPLC SEPARATION OF SEVERAL FREEALPHA-AMINO-ACIDS, Analytical letters, 26(8), 1993, pp. 1799-1818
Citations number
11
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
26
Issue
8
Year of publication
1993
Pages
1799 - 1818
Database
ISI
SICI code
0003-2719(1993)26:8<1799:TUOA3S>2.0.ZU;2-O
Abstract
A 3-factor statistical mixture design and a 2-factor chromatographic r esponse function were used in the solvent optimization of the RP-HPLC separation of a mixture containing free histidine, methionine, tyrosin e, phenylalanine and trypthphan. The influences of phosphoric acid, me thanol and acetonitrile on the separation were compared. A solvent con dition resulting in a satisfactory separation of the amino acid mixtur e was predicted.