2-IMINOTHIOLANE - A REAGENT FOR THE INTRODUCTION OF SULFHYDRYL-GROUPSINTO OLIGOSACCHARIDES DERIVED FROM ASPARAGINE-LINKED GLYCANS

Authors
TARENTINO AL PHELAN AW PLUMMER TH
Citation
Al. Tarentino et al., 2-IMINOTHIOLANE - A REAGENT FOR THE INTRODUCTION OF SULFHYDRYL-GROUPSINTO OLIGOSACCHARIDES DERIVED FROM ASPARAGINE-LINKED GLYCANS, Glycobiology, 3(3), 1993, pp. 279-285
Citations number
23
Categorie Soggetti
Biology
Journal title
Glycobiology → ACNP
ISSN journal
09596658
Volume
3
Issue
3
Year of publication
1993
Pages
279 - 285
Database
ISI
SICI code
0959-6658(1993)3:3<279:2-ARFT>2.0.ZU;2-5
Abstract
A facile method for introducing reactive sulphydryl groups into oligos accharides was developed. 1-Amino-oligosacchandes generated from aspar agine-linked glycans by peptide-N4(N-acetyl-beta-D-glucosaminyl) aspar agine amidase (PNGase F) digestion were monitored by high-performance anion-exchange chromatography with pulsed amperometric detection and d erivatized under optimal conditions with 2-iminothiolane-HCl. The resu lting mercapto-butyramido oligosaccharides, which were obtained in hig h yield, were alkylated with a fluorescent reagent and used to selecti vely assay for endoglycosidases that hydrolyse di-N-acetylchitobiose l inkages.