DEPENDENCE OF THE INFLUENZA-VIRUS HEMAGGLUTININ CONTENT DETERMINATIONBY SINGLE RADIAL IMMUNODIFFUSION UPON DETERGENT AND THE TEST SYSTEM USED

The assay systems for single radial immunodiffusion (SRD) represented by standard reagents of antigens and monospecific antisera to A and B influenza virus haemagglutinin and prepared in two different countries were used for elucidation of the influence of monospecific antisera a nd some ionic and nonionic detergents on the determination Of hemagglu tinin content in the homologous (antigen and antiserum from the same c ountry) and heterologous (antigen and antiserum from different countri es) assay systems. A high specificity of SRD reaction excluding substi tution of antiserum from one country for analogous preparation from an other as well as detergent interchangeability were shown.