The study of ancient biomolecules in preserved wheat remains could pro
vide information on both the initial development of agriculture and it
s subsequent trajectories and spread in relation to changing human soc
iety. We have detected ancient DNA in extracts prepared from charred,
waterlogged and mineralized wheat seeds, and have used the polymerase
chain reaction to study specific genetic regions of the ancient DNA pr
eserved in Triticum spelta from Danebury, UK and T. dicoccum from Assi
ros, Greece. We discuss future developments in the biomolecular archae
ology of wheat, including the possibility of a genetic test that may e
nable tetraploid and hexaploid wheat remains to be distinguished by an
cient DNA analysis.