RECOGNITION OF LIPOSOME-BOUND ANTIGENS BY ANTIPEPTIDE ANTIBODY

In order to clarify the effects of the differences in physical states of antigens on recognition by antibodies in immunoassays, the binding characteristics of an antipeptide polyclonal antibody to the peptide a nd the corresponding protein were studied. The reactivity in the immun oliposome assay (ILA), as well as in the double-antibody sandwich ELIS A, was identical to that in the solution. These results indicate that the conformation of liposome-bound antigen is changed little by coupli ng to liposomes and is almost the same as that of the native antigen i n the liquid phase. It is desirable to assay by double-antibody sandwi ch ELISA or ILA to detect native proteins, and the latter is very easi ly performed.