SEPARATION OF AMINOACIDATO RUTHENIUM(II) COMPLEXES BY ION-PAIR CHROMATOGRAPHY

The chromatographic conditions for analytical separation of (eta6-C6H6 ) ruthenium(II) complexes with amino acid and dipeptide ligands have b een investigated in dependence on their molecular structure. Different chromatographic phases were tested and the efficiency parameters were determined by the reversed-phase ion-pair technique. For the enantiom eric species L-penicillaminato ruthenium(II) complexes cis- and trans- [(eta6-C6H6)Ru(L-Pen)H-1]2Cl2 and the reaction byproducts a complete chromatographic separation has been obtained using sodium dodecylsulph ate as an ion-pair reagent. For the purification procedure of the L-pe nicillaminato ruthenium(II) complexes, in order to characterize the se parated pure complexes by H-1-NMR spectroscopy, a preparative separati on has been carried out using the volatile trifluoroacetic acid as an ion-pair reagent.