TLC-SCREENING OF IMPORTANT PHARMACEUTICAL SUBSTANCES USING FLUORESCENCE DETECTION

Observation of fluorescence reactions on TLC plates is a valuable addi tional tool within the scope of screening procedures for many toxicolo gically relevant substances commonly encountered in clinical- and fore nsic-toxicological analysis. The reactions are based on native fluores cence without any treatment and on reactions obtained with an approved derivatization procedure. Due to the enorinous sensitivity of the flu orescence detection, the method is also applicable to very low concent rations and small amounts of biological materials. The procedures desc ribed in this article have proven their high pragmatic usefulness in m any practical cases.