IMMOBILIZATION OF GLUTARYL-7-ACA ACYLASE ON AMINOALKYLATED POLYACRYLIC SUPPORTS

A procedure is described for the immobilization of glutaryl-7-ACA acyl ase from recombinant E. coli on Amberlite XAD7 modified by transamidat ion with I,2-diaminoethane and activated with glutaraldehyde. The dete rmination of the optimal operative conditions such as enzyme loading, effect of pH, temperature, and product inhibition is described for the hydrolysis of 7-beta-(4-carboxybutanamido) cephalosporanic acid (glut aryl-7-ACA) to give 7-aminocephalosporanic acid (7-ACA). The inactivat ion kinetics of the immobilized preparations determined in a continuou s fixed-bed reactor are also reported. (C) 1997 by Elsevier Science In c.