A NEW METHOD FOR THE CONCENTRATION OF CRYPTOSPORIDIUM OOCYSTS FROM WATER

Authors
VESEY G SLADE JS BYRNE M SHEPHERD K FRICKER CR
Citation
G. Vesey et al., A NEW METHOD FOR THE CONCENTRATION OF CRYPTOSPORIDIUM OOCYSTS FROM WATER, Journal of Applied Bacteriology, 75(1), 1993, pp. 82-86
Citations number
7
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Journal of Applied Bacteriology → ACNP
ISSN journal
00218847
Volume
75
Issue
1
Year of publication
1993
Pages
82 - 86
Database
ISI
SICI code
0021-8847(1993)75:1<82:ANMFTC>2.0.ZU;2-S
Abstract
A novel method for the concentration of Cryptosporidium oocysts from w ater has been developed, based upon the precipitation of calcium carbo nate. A 10 1 water sample is treated by adding solutions of calcium ch loride and sodium bicarbonate and raising the pH value to 10 with sodi um hydroxide. Crystals of calcium carbonate form and enmesh particles in the Cryptosporidium oocyst size range. The crystals are allowed to settle, the supernatant fluid is discarded and the calcium carbonate p recipitate dissolved in sulphamic acid. The sample can be concentrated further by centrifugation. Recoveries of oocysts from seeded samples of deionized, tap and river water were in excess of 68%.