Sc. Beu et al., FOURIER-TRANSFORM ELECTROSPRAY INSTRUMENTATION FOR TANDEM HIGH-RESOLUTION MASS-SPECTROMETRY OF LARGE MOLECULES, Journal of the American Society for Mass Spectrometry, 4(7), 1993, pp. 557-565
Mass spectrometry instrumentation providing unit resolution and 10-ppm
mass accuracy for molecules larger than 10 kDa was first reported in
1991. This instrumentation has now been improved with a 6.2-T magnet r
eplacing that of 2.8 T, a more efficient vacuum system, ion injection
with controlled ion kinetic energies, accumulated ion trapping with an
open-cylindrical ion cell, acquisition of 2M data points, and updated
electrospray apparatus. The resulting capabilities include resolving
power of 5 x 10(5) for a 29-kDa protein, less than 1-ppm mass measurin
g error, and dissociation of protein molecular ions to produce dozens
of fragment ions whose exact masses can be identified from their mass-
to-charge ratio values and isotopic peak spacing.