INTERLEUKIN-11 INDUCES RAPID PKC ACTIVATION AND CYTOSOLIC TO PARTICULATE TRANSLOCATION OF ALPHA-PKC AND BETA-PKC ISOFORMS IN HUMAN ERYTHROLEUKEMIA K562 CELLS

Interleukin-11 (IL-11) is a pleiotropic cytokine which regulates the g rowth of hematopoietic progenitor cells and activates platelet maturat ion. Previous studies have shown that, IL-11 activates a set of signal ing cascades involving the JAK/STAT and Raf/MAPK pathways. The purpose of the current studies was to obtain evidence about the possible invo lvement of PKC in the IL-11 signaling pathway. Evidence presented in t his report suggests that IL-11 stimulates rapid PKC activation and mar kedly induces cytosolic to particulate (membrane) association of alpha and beta PKC isoforms. These findings provide preliminary evidence th at PKC may be involved in the IL-11 signaling cascade. (C) 1997 Academ ic Press.