THE SCHIZOSACCHAROMYCES-POMBE GMS1(-GALACTOSE TRANSPORTER HOMOLOG REQUIRED FOR PROTEIN GALACTOSYLATION() GENE ENCODES AN UDP)

Citation
M. Tabuchi et al., THE SCHIZOSACCHAROMYCES-POMBE GMS1(-GALACTOSE TRANSPORTER HOMOLOG REQUIRED FOR PROTEIN GALACTOSYLATION() GENE ENCODES AN UDP), Biochemical and biophysical research communications, 232(1), 1997, pp. 121-125
Citations number
25
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
232
Issue
1
Year of publication
1997
Pages
121 - 125
Database
ISI
SICI code
0006-291X(1997)232:1<121:TSGTHR>2.0.ZU;2-V
Abstract
In a previous study, we isolated a Schizosaccharomyces pombe mutant de fective in protein galactosylation (Takegawa, K., Tanaka, N., Tabuchi, M. and Iwahara, S. (1996) Biosci. Biochem. Biotech. 60, 1156-1159), F rom an S. pombe genomic library, we cloned the gms1(+) gene which rest ored the galactosylation of cell wall glycoproteins. Gms1 protein shar es significant sequence similarity with human UDP-galactose and murine CMP-sialic acid transporters, The fission yeast strains deleted for t he gms1(+) gene lacked galactose residues in sell surface glycoprotein s and were significantly decreased in UDP-galactose transport activity , These results showed that the gms1(+) encodes an UDP-galactose trans porter, and this protein appears to be an essential role for the incor poration of UDP-galactose into the lumen of Golgi in S. pombe. (C) 199 7 Academic Press.