M. Tabuchi et al., THE SCHIZOSACCHAROMYCES-POMBE GMS1(-GALACTOSE TRANSPORTER HOMOLOG REQUIRED FOR PROTEIN GALACTOSYLATION() GENE ENCODES AN UDP), Biochemical and biophysical research communications, 232(1), 1997, pp. 121-125
In a previous study, we isolated a Schizosaccharomyces pombe mutant de
fective in protein galactosylation (Takegawa, K., Tanaka, N., Tabuchi,
M. and Iwahara, S. (1996) Biosci. Biochem. Biotech. 60, 1156-1159), F
rom an S. pombe genomic library, we cloned the gms1(+) gene which rest
ored the galactosylation of cell wall glycoproteins. Gms1 protein shar
es significant sequence similarity with human UDP-galactose and murine
CMP-sialic acid transporters, The fission yeast strains deleted for t
he gms1(+) gene lacked galactose residues in sell surface glycoprotein
s and were significantly decreased in UDP-galactose transport activity
, These results showed that the gms1(+) encodes an UDP-galactose trans
porter, and this protein appears to be an essential role for the incor
poration of UDP-galactose into the lumen of Golgi in S. pombe. (C) 199
7 Academic Press.