ADSORPTION-DESORPTION OF HUMAN SERUM-ALBUMIN AT THE SURFACE OF POLY(LACTIC ACID) NANOPARTICLES PREPARED BY A SOLVENT EVAPORATION PROCESS

Biocompatible and biodegradable nanoparticles of poly(lactic acid) (10 0% L-lactic units = PLA) were prepared by an emulsion, microfluidizati on, and solvent evaporation method using human serum albumin (HSA) as a surface agent. A radiolabeling technique was employed to quantify th e serum albumin bound to the nanoparticles and to measure its desorpti on kinetics in various media at 22-degrees-C and 37-degrees-C (phospha te buffer pH 7.4, serum albumin 40 g/L in phosphate buffer pH 7.4 and fetal calf serum). The amount of serum albumin bound to the nanopartic les was found to be a linear function of 1/D (where D is the nanoparti cle mean diameter) and was related to the total developed area of the nanoparticles. The adsorption/desorption behavior of serum albumin at the surface of the nanoparticles suggested a multi-layer adsorption mo del. Moreover, a part of the serum albumin molecules was irreversibly bound regardless of the incubation conditions. Consequently, the class ical Langmuirian theories of equilibria could not be applied. (C) 1993 John Wiley & Sons, Inc.