RECONSTITUTION INTO LIPOSOMES OF THE TONOPLAST ATP-DEPENDENT AND PYROPHOSPHATE-DEPENDENT PROTON PUMPS FROM RUBUS-HISPIDUS CELL-CULTURES

The ATP- and pyrophosphate-dependent proton pumps from tonoplast-enric hed vesicles prepared from Rubus hispidus cell cultures were solubiliz ed in the presence of polyoxyethylene(9,10)p-t-octylphenol (Triton X-1 00) and reconstituted into liposomes of soybean phospholipids, using B io-Beads SM-2 to remove the detergent. The specific activity of the tw o pumps was greatly increased by the solubilization-reconstitution pro cedure. Identical characteristics were found for pyrophosphate-depende nt proton transport in native and reconstituted vesicles. On the other hand, the ATP-dependent proton transport of the reconstituted vesicle s was no longer inhibited by KNO3.