FORMATE BOUND TO CYTOCHROME-OXIDASE CAN BE REMOVED BY CYANIDE AND BY REDUCTION

Authors
CHANG KT PALMER G
Citation
Kt. Chang et G. Palmer, FORMATE BOUND TO CYTOCHROME-OXIDASE CAN BE REMOVED BY CYANIDE AND BY REDUCTION, Biochimica et biophysica acta. Bioenergetics, 1277(3), 1996, pp. 237-242
Citations number
17
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Bioenergetics → ACNP
ISSN journal
00052728
Volume
1277
Issue
3
Year of publication
1996
Pages
237 - 242
Database
ISI
SICI code
0005-2728(1996)1277:3<237:FBTCCB>2.0.ZU;2-F
Abstract
Using C-14-radiolabeled formate we have found that the rapid form of o xidized cytochrome oxidase can bind up to 1 mol of formate. Treatment of this formate-ligated enzyme with excess cyanide releases 97% of the radiolabel while reduction of formate-labeled enzyme with NADH + ruth enium releases 80-85% of the radioactivity. These data are most simply interpreted by assuming that formate binds to the heme iron of cytoch rome a(3).