SEPARATION OF PHAGOCYTIC LEUKOCYTES FROM THE PERIPHERAL-BLOOD OF CHICKENS

Authors
MILLS JN WILCOX GE
Citation
Jn. Mills et Ge. Wilcox, SEPARATION OF PHAGOCYTIC LEUKOCYTES FROM THE PERIPHERAL-BLOOD OF CHICKENS, Avian pathology, 22(2), 1993, pp. 343-352
Citations number
29
Categorie Soggetti
Veterinary Sciences
Journal title
Avian pathology → ACNP
ISSN journal
03079457
Volume
22
Issue
2
Year of publication
1993
Pages
343 - 352
Database
ISI
SICI code
0307-9457(1993)22:2<343:SOPLFT>2.0.ZU;2-P
Abstract
Phagocytic leukocytes were separated from the peripheral blood of chic kens using a one-step Percoll density gradient technique. Heterophils recovered from two fractions of the gradient were of 96.9 to 99.8% pur ity and were fully viable and functional, as demonstrated by their cap acity to phagocytose latex beads and staphylococci. Adherent mononucle ar cells were cultured from specific gradient fractions and shown to p hagocytose staphylococci.