EXPRESSION OF THE MAIN ANTIGENS OF YERSIN IA-PESTIS CELLS CULTIVATED OF 37-DEGREES-C IN CULTURE MEDIA HAVING DIFFERENT COMPOSITION

To improve the technological process of the preparation of vaccines fr om Y. pestis, the influence of different cultivation conditions on the antigenic composition of bacterial cells was studied. The study of th e influence of pH (6.8-7.2), the volume of the culture medium in a fla sk (25-100 ml), the concentration of amino nitrogen (100-200 mg%) on t he growth and phenotypic expression of Y. pestis EV NIIEG antigen s at 37-degrees-C was studied according to the plan of a complete factor e xperiment. The data obtained in this study demonstrated the nonlinear dependence of the yield of viable cells on the factors chosen for the process. The presence of F1 and Pst was detected in all tested culture s. The use of the correlation field method made it possible to reveal that the production of antigen pH6 depended on the increased aeration and decreased viability index of the culture. For the production of Ps t reverse dependence was revealed.