HIGHLY SENSITIVE AND HOMOGENEOUS METHOD F OR THE DETERMINATION OF ANTIBODIES AND ANTIGENS BY MEANS OF LIPOSOMES, MONOCLONAL-ANTIBODIES AND COMPLEMENT

Authors
SKOPINSKAYA SN TAMULEVICH YA YARKOV SP ZLOBIN VN KALININ YT
Citation
Sn. Skopinskaya et al., HIGHLY SENSITIVE AND HOMOGENEOUS METHOD F OR THE DETERMINATION OF ANTIBODIES AND ANTIGENS BY MEANS OF LIPOSOMES, MONOCLONAL-ANTIBODIES AND COMPLEMENT, Zurnal mikrobiologii, epidemiologii i immunobiologii, (1), 1993, pp. 77-82
Citations number
15
Categorie Soggetti
Microbiology,Immunology,"Public, Environmental & Occupation Heath
Journal title
Zurnal mikrobiologii, epidemiologii i immunobiologii → ACNP
ISSN journal
03729311
Issue
1
Year of publication
1993
Pages
77 - 82
Database
ISI
SICI code
0372-9311(1993):1<77:HSAHMF>2.0.ZU;2-9
Abstract
A simple and sensitive method for the determination of Francisella tul arensis antigen and antibodies to this antigen by means of the complem ent lysis of liposomes sensitized with F. tularensis lipopolysaccharid e (LPS) is proposed. The possibility of participation of monoclonal an tibodies (McAb) in the complement-dependent lysis of liposomes has bee n studied. The method permits the detection of 50-100 ng/ml of soluble LPS antigen in the presence of antiserum or McAb IgG F-B11-x and of 5 0-10 ng/ml of it in the presence of IgG F-B11-x and anti-IgG within 90 minutes.