S. Oldfield et Cg. Proud, PHOSPHORYLATION OF ELONGATION FACTOR-II FROM THE LEPIDOPTERAN INSECT,SPODOPTERA-FRUGIPERDA, FEBS letters, 327(1), 1993, pp. 71-74
In mammalian cells, protein synthesis can be regulated at the level of
elongation by the phosphorylation of elongation factor 2 (eEF-2) by a
highly specific Ca2+/calmodulin-dependent kinase. In this report, we
show that eEF-2 from a cell line derived from the insect, Spodoptera f
rugiperda, is a substrate for mammalian eEF-2 kinase and that phosphor
ylation is Ca2+-dependent. Furthermore, two-dimensional peptide mappin
g shows that the kinase phosphorylates the same sites in Spodoptera eE
F-2 as those phosphorylated in the rabbit protein. However, we were un
able to detect an eEF-2 kinase in Spodoptera cells.