S. Lang et P. Loidl, IDENTIFICATION OF PROTEINS IMMUNOLOGICALLY RELATED TO VERTEBRATE LAMINS IN THE NUCLEAR MATRIX OF THE MYXOMYCETE PHYSARUM-POLYCEPHALUM, European journal of cell biology, 61(1), 1993, pp. 177-183
Lamin-like proteins have been identified as components of the nuclear
matrix of the myxomycete Physarum polycephalum. A 67 kDa homologue was
detected by immunoblotting of nuclear matrix proteins with affinity-p
urified anti-lamin antibodies of human autoimmune sera. A 65 kDa lamin
B homologue was identified with polyclonal antibodies against turkey
erythrocyte lamin B in nuclei and nuclear matrix of Physarum amoebae a
nd plasmodia. Indirect immunofluorescence microscopy revealed that the
65 and 67 kDa proteins are localized in the nuclear lamins of plasmod
ial nuclei.