AMPLIFICATION OF INTEGRATED BOVINE GROWTH -HORMONE BGH-TRANSGENE BY MEANS OF THE PCR REACTION

Conditions of the PCR amplification of unique fragments of bovine grow th hormone (bGH) were found that allow reliable identification of tran sgenic rabbits for this gene. An integrated construction containing a part of MT promoter of metallothioneine gene and chromosomal bGH gene was amplified. Two of 46 newborn lambs were found to be transgenic for the bGH gene by using primers BG3 and BG4 for amplification of the ge ne bGH fragment and its following restriction with pVUII. We managed t o whow the possibility of successful amplification of growth hormones genes of pig and sheep using primers BG3 and BG8, corresponding respec tively to the beginning and the end of the bGH gene.