Giemsa C-banding is applied for the first time in Capsicum, allowing p
reliminary karyotype differentiation of six diploid species. Compariso
n of interphase nuclei and heterochromatic C-bands reveals striking di
fferences between taxa and contributes to their taxonomic grouping. Th
erefore, C-banding appears to be a powerful tool for the cytogenetics
and karyosystematics of the genus. Banding patterns are characterized
by the omnipresence of centromeric bands and a variable number of smal
ler to larger distal bands, with the addition of intercalary bands in
some cases. Satellites are always C-positive. Relationships between sp
ecies and possible trends of karyotype evolution are discussed, with s
pecial reference to the origin of x = 13 from x = 12 and the increase
of heterochromatin, regarded as advanced features.