EFFECT OF INFLUENZA-A VIRUS ON CILIARY ACTIVITY AND DYE TRANSPORT FUNCTION IN THE CHINCHILLA EUSTACHIAN-TUBE

Previous studies have shown that influenza A virus inoculated via a tr ansbullar approach can cause severe disorders of the chinchilla eustac hian tube. In this study, we inoculated chinchillas both intranasally and transbullarly with influenza A virus (A/Alaska/6/77) to assess the effect of this virus on both the ciliary activity of eustachian tube epithelium and eustachian tube transport function. Data indicated that while the nature of the major lesions was indistinguishable, the diff ering routes of inoculation did influence the time course and initial location of virus-induced histopathology. Analysis of ciliary beat fre quency and dye transport function indicated a maximal decrease approxi mately 7 to 14 days postinoculation, with return to normal function by 28 days postinoculation. Light and transmission electron microscopic observations of the evolution of histopathology and return to normal h istology correlated well with functional assessment data.