Dw. Jackson et al., CELL-SURVIVAL AFTER TRANSPLANTATION OF FRESH MENISCAL ALLOGRAFTS - DNA-PROBE ANALYSIS IN A GOAT MODEL, American journal of sports medicine, 21(4), 1993, pp. 540-550
Fibrochondrocytes synthesize and maintain the extracellular matrix res
ponsible for the distinctive material and structural properties of a n
ormal meniscus. Viable meniscal cells are believed to be necessary for
the long-term maintenance of these properties in meniscal allografts.
The purpose of this study was to determine if the donor cells (fibroc
hondrocytes) survive after a fresh meniscal allograft transplantation.
A DNA probe technique was used to clearly distinguish the DNA pattern
s in donor cells from the host cells in the Spanish goat. No remaining
donor DNA could be demonstrated at 4 weeks in transplanted meniscal t
issue; it was all of host origin. The host DNA content at 4 weeks appr
oached or exceeded the amount present in the contralateral control men
iscus. Clinical significance: The results of this study demonstrate th
at viable cells in medial meniscal allografts transplanted from one an
imal to another do not survive. Host cells rapidly repopulate the tran
splanted meniscus. There is no evidence these new cells will maintain
on a long-term basis the extracellular matrix of the meniscus. The evi
dence in this paper, that the fibrochondrocytes do not survive transpl
antation, suggests further justification is necessary for using grafts
with living cells. Allografts with living cells have an increased exp
ense, more complicated surgical logistics, and have a higher potential
risk of disease transmission.