SEQUENCE OF A 17 KDA VACUOLAR H-ATPASE PROTEOLIPID SUBUNIT FROM INSECT MIDGUT AND MALPIGHIAN TUBULES()

Authors
PIETRANTONIO PV GILL SS
Citation
Pv. Pietrantonio et Ss. Gill, SEQUENCE OF A 17 KDA VACUOLAR H-ATPASE PROTEOLIPID SUBUNIT FROM INSECT MIDGUT AND MALPIGHIAN TUBULES(), Insect biochemistry and molecular biology, 23(6), 1993, pp. 675-680
Citations number
32
Categorie Soggetti
Entomology,Biology
Journal title
Insect biochemistry and molecular biology → ACNP
ISSN journal
09651748
Volume
23
Issue
6
Year of publication
1993
Pages
675 - 680
Database
ISI
SICI code
0965-1748(1993)23:6<675:SOA1KV>2.0.ZU;2-1
Abstract
A 0.4 kb polymerase chain reaction (PCR) product obtained from cDNA ma de from the midgut and Malpighian tubules of fifth instar larvae of He liothis virescens was used to screen a larval midgut and Malpighian tu bules cDNA library. Four clones were obtained, one of 1.9 kb and other s of 1.4 kb. The 1.9 kb clone encodes a 17.2 kDa protein which is high ly homologous to other vacuolar ATPases proteolipids. Putative N-glyco sylation and DCCD binding sites were observed at amino acid residues 8 3 and 139, respectively.