IDENTIFICATION OF HUMAN DNA IN COMPLEX BIOLOGICAL SAMPLES USING THE ALU POLYMERASE CHAIN-REACTION

Authors
TSONGALIS GJ COLEMAN WB ESCH GL SMITH GJ KAUFMAN DG
Citation
Gj. Tsongalis et al., IDENTIFICATION OF HUMAN DNA IN COMPLEX BIOLOGICAL SAMPLES USING THE ALU POLYMERASE CHAIN-REACTION, Journal of forensic sciences, 38(4), 1993, pp. 961-967
Citations number
16
Categorie Soggetti
Medicine, Legal
Journal title
Journal of forensic sciences → ACNP
ISSN journal
00221198
Volume
38
Issue
4
Year of publication
1993
Pages
961 - 967
Database
ISI
SICI code
0022-1198(1993)38:4<961:IOHDIC>2.0.ZU;2-J
Abstract
Alu-Polymerase chain reaction (PCR) was used to amplify human DNA from complex mixed sources of DNA. Amplification of human DNA sequences by Alu-PCR could be accomplished in samples containing low concentration s of template in the presence of excess heterologous DNA sequences. Th us, sensitivity and specificity are maintained in complex DNA mixtures allowing positive identification of the presence of human DNA sequenc es by this technique.