MOTOR CORTICAL ACTIVITY DURING VOLUNTARY GAIT MODIFICATIONS IN THE CAT .1. CELLS RELATED TO THE FORELIMBS

1. The discharge patterns of 91 identified pyramidal tract neurons (PT Ns), located within the forelimb region of area 4 of the cat motor cor tex, were recorded during the voluntary modifications of gait needed t o step over obstacles attached to a moving treadmill belt. Recordings were made simultaneously from flexor and extensor muscles acting aroun d the shoulder, elbow, wrist, and digits of the forelimb contralateral to the recording site. 2. Analysis of the changes in electromyographi c (EMG) activity during the gait modification showed increases in the activity of most flexor muscles of the shoulder and elbow, as well as in the wrist and digit dorsiflexors, when the contralateral forelimb w as the first to pass over the obstacle. This period of augmented activ ity could be subdivided into two parts: one associated with the initia l flexion of the limb that was needed to bring it above and over the o bstacle (phase I), and the second associated with increased wrist dors iflexor muscle activity before foot contact (phase II). 3. The dischar ge frequency of a total of 57/91 (63%) of the recorded PTNs was signif icantly increased during the gait modification when the limb contralat eral to the recording site was the first to step over the obstacle; si x of these neurons also showed a significant decrease in their dischar ge in a different part of the step cycle. In a further 21/91 (23%) neu rons, discharge frequency was only decreased, whereas the remaining 13 /91 (14%) PTNs showed similar patterns of activity both during control walking and during the gait modifications. 4. Most of those neurons ( 47/57) in which significant increases in firing frequency were observe d, discharged maximally during the period of increased activity of the physiological flexor muscles. Twenty-three of these cells (23/47) dis charged maximally in phase I, and 12 (12/47) in phase II. A third popu lation of PTNS (12/47) started to increase their discharge in the stan ce phase of the step cycle immediately preceding the modified cycle. S even (7/57) PTNs increased their discharge during the stance phase of the modified cycle, and the remaining three could not be classified as being preferentially related to any one part of the step cycle. 5. Th e frequency modulation of 41/57 PTNs was less when the leg contralater al to the recording site was the second to encounter the obstacle. In many neurons there was also an appreciable change in the time in the s tep cycle that peak discharge occurred. These changes in amplitude and timing paralleled the changes observed in the temporal relationships of the muscles. 6. Receptive fields could be determined for 71 of the 73 PTNs tested. Altogether 54/71 PTNs had receptive fields that includ ed the forepaw, and 55/71 PTNs were activated by light brushing of the skin surface (cutaneous receptive fields). In most cases neither the discharge of the cell during control walking, nor its discharge during the steps over the obstacles, could be explained, or predicted, on th e basis of the receptive field. In the extreme case, PTNs with recepti ve fields restricted to the plantar surface of the paw discharged duri ng the swing phase of the gait modification. It is suggested that in m ost cases the increase in cell discharge is due to visually triggered central, rather than peripheral, inputs. 7. Comparison of the relative time of activation of motor cortical neurons when the contralateral l imb led, and when it trailed, suggested that these PTNs could be divid ed into different populations of cells active at different times durin g the pit modification. It is suggested that each of these populations may regulate the activity of muscles active at different times in the step cycle by modulating the activity of interneurons that either for m part of, or that are influenced by, the central pattern generator fo r locomotion.