FERROCENE-MEDIATED THERMAL BIOSENSOR

A biosensor that combines electrochemistry and calorimetry was investi gated. Using this sensor, glucose measurements were performed by emplo ying glucose oxidase together with ferrocene as electron-transfer medi ator. The electrochemical reaction was accomplished by applying a volt age between a platinum column (working electrode), in contact with a c rushed reticulated vitreous carbon (RVC) matrix onto which glucose oxi dase was immobilized, and platinum wires (counter electrodes) located at the inlet and outlet of the column. For detection, the thermal sign al generated by the redox reaction was measured as opposed to measurin g the electrochemical signal. By using this method, a linear range of glucose concentration up to 20 mmol 1-1 was achieved.