DETERMINATION OF L-MALATE IN WINE BY FLOW-INJECTION WITH CO-IMMOBILIZED MALATE-DEHYDROGENASE OXALOACETATE DECARBOXYLASE

Authors
KIBA N OGUCHI M FURUSAWA M
Citation
N. Kiba et al., DETERMINATION OF L-MALATE IN WINE BY FLOW-INJECTION WITH CO-IMMOBILIZED MALATE-DEHYDROGENASE OXALOACETATE DECARBOXYLASE, Talanta, 40(8), 1993, pp. 1163-1165
Citations number
12
Journal title
Talanta
ISSN journal
00399140 → ACNP
Volume
40
Issue
8
Year of publication
1993
Pages
1163 - 1165
Database
ISI
SICI code
0039-9140(1993)40:8<1163:DOLIWB>2.0.ZU;2-5
Abstract
A method for determination of L-Malate in wine is described. Malate de hydrogenase and oxaloacetate decarboxylase were immobilized on poly (v inyl alcohol) beads and incorporated in a flow-injection system with f luorescence detection. Sample solution (50 mul) was injected into the carrier stream [4mM NAD+ in glycine buffer (pH 10.0)]. The fluorescenc e based on the NADH formed could be directly related to the amount of malate. The calibration graph was linear over the range 0.4-300 muM. T he detection limit was 0.2 muM. Sampling throughout was 25 samples/hr.