CHARACTERIZATION OF AN 18-KILODALTON BRUCELLA CYTOPLASMIC PROTEIN WHICH APPEARS TO BE A SEROLOGICAL MARKER OF ACTIVE INFECTION OF BOTH HUMAN AND BOVINE BRUCELLOSIS
Fa. Goldbaum et al., CHARACTERIZATION OF AN 18-KILODALTON BRUCELLA CYTOPLASMIC PROTEIN WHICH APPEARS TO BE A SEROLOGICAL MARKER OF ACTIVE INFECTION OF BOTH HUMAN AND BOVINE BRUCELLOSIS, Journal of clinical microbiology, 31(8), 1993, pp. 2141-2145
Some anticytoplasmic protein monoclonal antibodies (MAbs) from mice im
munized by infection with Brucella ovis cells have been obtained. One
of these MAbs, BI24, was used to purify by immunoaffinity a protein wi
th a pl of 5.6 and a molecular mass of 18 kDa. This protein was presen
t in all of the rough and smooth Brucella species studied, but it coul
d not be detected in Yersinia enterocolitica 09. Three internal peptid
es of this protein were partially sequenced; no homology with other ba
cterial proteins was found. The immunogenicity of the 18-kDa protein w
as studied with both human and bovine sera by a capture enzyme-linked
immunosorbent assay system with MAb BI24.