CHARACTERIZATION OF AN 18-KILODALTON BRUCELLA CYTOPLASMIC PROTEIN WHICH APPEARS TO BE A SEROLOGICAL MARKER OF ACTIVE INFECTION OF BOTH HUMAN AND BOVINE BRUCELLOSIS

Authors
GOLDBAUM FA LEONI J WALLACH JC FOSSATI CA
Citation
Fa. Goldbaum et al., CHARACTERIZATION OF AN 18-KILODALTON BRUCELLA CYTOPLASMIC PROTEIN WHICH APPEARS TO BE A SEROLOGICAL MARKER OF ACTIVE INFECTION OF BOTH HUMAN AND BOVINE BRUCELLOSIS, Journal of clinical microbiology, 31(8), 1993, pp. 2141-2145
Citations number
23
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
31
Issue
8
Year of publication
1993
Pages
2141 - 2145
Database
ISI
SICI code
0095-1137(1993)31:8<2141:COA1BC>2.0.ZU;2-Q
Abstract
Some anticytoplasmic protein monoclonal antibodies (MAbs) from mice im munized by infection with Brucella ovis cells have been obtained. One of these MAbs, BI24, was used to purify by immunoaffinity a protein wi th a pl of 5.6 and a molecular mass of 18 kDa. This protein was presen t in all of the rough and smooth Brucella species studied, but it coul d not be detected in Yersinia enterocolitica 09. Three internal peptid es of this protein were partially sequenced; no homology with other ba cterial proteins was found. The immunogenicity of the 18-kDa protein w as studied with both human and bovine sera by a capture enzyme-linked immunosorbent assay system with MAb BI24.