ACCUMULATION OF ADENINE AND GUANINE AS CU-CARBON ELECTRODES FOLLOWED BY ANODIC-STRIPPING VOLTAMMETRY( COMPOUNDS AT GLASSY)

This paper deals with the electrochemical anodic stripping of purine b ases, such as adenine and guanine, in Cu2+ solution at glassy carbon e lectrodes (GCE). It was found that Cu2+ was reduced to Cu+ around 0 V vs. SCE and the generated Cu+ reacted with adenine and guanine to accu mulate on the GCE as an insoluble compound. The base-Cu+ compound accu mulated on the GCE was redissolved by the reoxidation of Cu+ to Cu2+ a t ca. 0.4 V and the concentration of the base in the vicinity of the G CE was increased, thus giving a large oxidation current for the base. Utilizing this stripping process, the linear calibration curves of ade nine and guanine were obtained in the range 0.5-8 AM. When the pre-ele ctrolysis period for accumulation was 8 min, 0.2 muM guanine was detec table by anodic stripping voltammetry.