SURFACE COATING - NO IMPROVEMENT OF SHEAR-STRESS RESISTANCE OF ANCHORAGE-DEPENDENT MAMMALIAN-CELLS

Authors
LUDWIG A WEIDEMANN R TOMECZKOWSKI J KRETZMER G
Citation
A. Ludwig et al., SURFACE COATING - NO IMPROVEMENT OF SHEAR-STRESS RESISTANCE OF ANCHORAGE-DEPENDENT MAMMALIAN-CELLS, Biotechnology techniques, 7(7), 1993, pp. 411-416
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
7
Issue
7
Year of publication
1993
Pages
411 - 416
Database
ISI
SICI code
0951-208X(1993)7:7<411:SC-NIO>2.0.ZU;2-H
Abstract
The growth of adherent Baby Hamster Kidney cells (BHK 21) on surfaces coated with different substances (FCS, Con. A, PHA, cholesterol, colla gen) was investigated. A positive influence on cell viability could on ly be observed when using a fetal calf serum (FCS) coat. To evaluate t he effects of coating on shear stress sensitivity, experiments in a ho me made flow chamber were carried out. For comparison further investig ations were conducted for several serum concentrations in the medium. The results demonstrate that the latter had a more beneficial effect o n shear stress resistance than coating.