OPTIMIZATION OF AN ELECTROPORATION PROCEDURE FOR KLUYVEROMYCES-LACTISTRANSFORMATION

Authors
RUSSELL C JARVIS A YU PL MAWSON J
Citation
C. Russell et al., OPTIMIZATION OF AN ELECTROPORATION PROCEDURE FOR KLUYVEROMYCES-LACTISTRANSFORMATION, Biotechnology techniques, 7(7), 1993, pp. 417-422
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
7
Issue
7
Year of publication
1993
Pages
417 - 422
Database
ISI
SICI code
0951-208X(1993)7:7<417:OOAEPF>2.0.ZU;2-F
Abstract
An electroporation method using a Bio-Rad Gene Pulser has been optimiz ed for introducing heterologous DNA into Kluyveromyces lactis yeasts. The plasmid pCR1, derived from a native Kluyveromyces plasmid, was use d to transform K. lactis. This plasmid produces a wheat alpha-amylase and contains both the biosynthetic marker URAA and G418 resistance gen es. Transformation was optimal at 4500 V/cm, 25 muF, and infinity OMEG A with 0.2 mug plasmid DNA. Transformation efficiencies in the range 1 0(4)-10(5) transformants/10(7) cells/mug DNA were obtained.