A SIMPLE METHOD FOR RECOVERING DNA FROM AGAROSE GELS USING GLASS-FIBER FILTERS

Authors
VERMA V QAZI GN
Citation
V. Verma et Gn. Qazi, A SIMPLE METHOD FOR RECOVERING DNA FROM AGAROSE GELS USING GLASS-FIBER FILTERS, Biotechnology techniques, 7(7), 1993, pp. 469-471
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
7
Issue
7
Year of publication
1993
Pages
469 - 471
Database
ISI
SICI code
0951-208X(1993)7:7<469:ASMFRD>2.0.ZU;2-Q
Abstract
A simple and reproducible procedure for the recovery of plasmid DNA is described. The method was standardised for the purification of plasmi ds from Gluconobacter oxydans ATCC9937 . The protocol is based on the use of glass microfibre filter paper for entrapment of DNA and its sub sequent recovery by an elution buffer. The method precludes the use of phenol and butanol for the removal of proteins and ethidium bromide r espectively, therefore, making the procedure inexpensive and gentle.