DETECTION OF THE C-PROTEIN GENE AMONG GROUP B-STREPTOCOCCI USING PCR

Aim-To develop a polymerase chain reaction (PCR) for the specific dete ction of the C protein gene in strains of group B Streptococcus. Metho ds-A single primer pair derived from the nucleotide sequence of the Ig A binding beta antigen of the C protein complex permitted the specific amplification of a 592 base pair DNA fragment from the C protein gene . After 35 cycles of amplification this product could be detected by a garose gel electrophoresis. Southern blot hybridisation confirmed that this product was the C protein gene. Results-PCR detected the C prote in gene in 75 (63%) of 119 strains of group B streptococci analysed. T he product was not detected in other Gram positive organisms, showing that this PCR assay was highly specific. The sensitivity of the assay was satisfactory to a dilution of 1 in 10 000 of extracted DNA. Conclu sions-The C protein of group B streptococci is associated with neonata l sepsis. The specific detection of the C protein gene by PCR may help identify which strains are likely to be associated with infection by the organism.