RADIOLABELING OF ANTICARCINOEMBRYONIC ANTIGEN (CEA) MONOCLONAL-ANTIBODY (MAB-BW-431 26) FOR CLINICAL-APPLICATIONS/

A method for labeling of anti-carcinoembryonic antigen (CEA) monoclona l antibody (MAb) BW 431/26 is described. For preparations of Tc-99m-an ti-CEA complex, a solution (1 ml) of tetrasodium-1,1,3.3-propanetetetr aphosphate dihydrate (2.7 mg), stannous chloride dihydrate (0.12 mg) a nd sodium chloride (0.2 mg) in 5 ml of 0.9% saline was added to a vial containing monoclonal antibody (2 mg) from mouse (MAb BW 431/26), D-g lucitol (2 mg) and sodium sulfate (2 mg) to obtain a clear solution. A quantitative labeling yield of the MAb BW 431/26 was achieved by addi tion of 5 mi (40.5 mCi, 1.5 GBq) technetium-99m-pertechnetate ((TcO4)- Tc-99m) generator eluate at room temperature within 10 minutes. The ra diochemical purity determined by ITLC (Gelman, SG) plates was > 95%.