PURIFICATION OF NUCLEOSIDE-5'-DIPHOSPHATES - A NEW ION-EXCHANGE METHOD

Authors
HUHN GF JENSEN JH PIERCE ME SCHLABACH EM
Citation
Gf. Huhn et al., PURIFICATION OF NUCLEOSIDE-5'-DIPHOSPHATES - A NEW ION-EXCHANGE METHOD, Separation science and technology, 28(11-12), 1993, pp. 1959-1970
Citations number
7
Categorie Soggetti
Engineering, Chemical","Chemistry Analytical
Journal title
Separation science and technology → ACNP
ISSN journal
01496395
Volume
28
Issue
11-12
Year of publication
1993
Pages
1959 - 1970
Database
ISI
SICI code
0149-6395(1993)28:11-12<1959:PON-AN>2.0.ZU;2-R
Abstract
Inosine- and uridine-5'-diphosphates can be purified by ion-exchange c hromatography utilizing a new ion-exchange method where Type 1 ion-exc hange resins and the absorbed nucleoside polyphosphates are changed to the basic form by rinsing the column with dilute sodium hydroxide and subsequently eluting with dilute hydrochloric acid and sodium chlorid e.