H. Louie et Vl. Chan, CLONING AND CHARACTERIZATION OF THE GAMMA-GLUTAMYL PHOSPHATE REDUCTASE GENE OF CAMPYLOBACTER-JEJUNI, MGG. Molecular & general genetics, 240(1), 1993, pp. 29-35
The gamma-glutamyl phosphate reductase gene, proA, of Campylobacter je
juni was isolated from a recombinant pBR322 clone. A HindIII fragment
of the insert containing the gene was subcloned into pUC19 and sequenc
ed in both orientations. The deduced amino acid sequence of gamma-glut
amyl phosphate reductase (EC 1.2.1.41) of C.jejuni exhibits 36.4% iden
tity to that of Escherichia coli and 36.0% identity to Serratia marces
cens. Two highly conserved regions in the amino acid sequence were ide
ntified from the alignment of the three available gamma-glutamyl phosp
hate reductase gene sequences. The gene was expressed from its own pro
moter and the transcription start site was mapped. The proline biosynt
hetic genes of C. jejuni are not located tandemly and thus differ in t
his respect from those of E. coli and S. marcescens, where gamma-gluta
myl phosphate reductase and gamma-glutamyl kinase (proB) are located i
n a single operon.