A NUCLEOTIDE SUBSTITUTION IN ONE OF THE BETA-TUBULIN GENES OF TRICHODERMA-VIRIDE CONFERS RESISTANCE TO THE ANTIMITOTIC DRUG METHYL BENZIMIDAZOLE-2-YL-CARBAMATE
Gh. Goldman et al., A NUCLEOTIDE SUBSTITUTION IN ONE OF THE BETA-TUBULIN GENES OF TRICHODERMA-VIRIDE CONFERS RESISTANCE TO THE ANTIMITOTIC DRUG METHYL BENZIMIDAZOLE-2-YL-CARBAMATE, MGG. Molecular & general genetics, 240(1), 1993, pp. 73-80
We characterized a Trichoderma viride strain that is resistant to the
antimitotic drug methyl benzimidazole-2-yl-carbamate (MBC). This speci
es has two beta-tubulin genes (tub1 and tub2) and by reverse genetics
we showed that a mutation in the tub2 gene confers MBC resistance in t
his strain. Comparison of the tub2 sequence of the mutant strain with
that of the wild type revealed that a single amino acid substitution o
f tyrosine for histidine at position 6 is responsible for the MBC tole
rance. Furthermore, we showed that this gene can be used as a homologo
us dominant selectable marker in T viride transformation. Both tubulin
genes were completely sequenced. They differ by 48 residues and the d
egree of identity between their deduced amino acid sequences is 86.3%.